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Fisher Scientific
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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
doi: 10.3791/62182
Figure Lengend Snippet: Outlines PCR primer sequences used for the detection of select genes involved in neonatal keratinocyte plasticity.
Article Snippet: Use GAPDH as an internal control ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Name of Material/ Equipment Company Catalog Number Comments/Description MCDB 153-LB basal media Sigma-Aldrich M7403 MCDB 153-LB basal media w/ HEPES buffer KSFM-scm ThermoFisher Scientific 17005042 Supplemented with 1% Penicillin/Streptomycin, 20 ng/ml EGF, 10 ng/ml basic fibroblast growth factor, 0.4% bovine serum albumin (BSA), and 4 μg/ml insulin Human Basic Fibroblast Growth Factor (hFGF basic/FGF2) Cell Signaling Technology 8910 Human Epidermal Growth Factor (hEGF) Cell Signaling Technology 8916 Human Insulin Millipore Sigma 9011-M Thermo Scientific™ Sterile Single Use Vacuum Filter Units Thermo Scientific 09-740-63D FITC-conjugated anti-integrinα6 Abcam ab30496 PE-conjugated anti-EGFR ( San Jose, CA; catalog number ) BD Pharmingen 555997 Dispase Sigma-Aldrich D4818 NEST Scientific 1-Well Cell Culture Chamber Slide, BLACK Walls on Glass Slide, 6/PK, 12/CS Stellar Scientific NST230111 P63 Thermo Scientific 703809 1:200 dilution Cytokeratin 14 Santa Cruz Biotechnology sc-53253 1:200 dilution All
Techniques: Cell Culture, SYBR Green Assay, Plasmid Preparation
Journal: Experimental and Therapeutic Medicine
Article Title: Expression and role of CaMKII and Cx43 in a rat model of post-stroke depression
doi: 10.3892/etm.2019.7782
Figure Lengend Snippet: RT-qPCR detection of CaMKII and Cx43 expression. The relative expression of CaMKII mRNA in the PSD and KN93 groups was significantly higher than that in the control group (both P<0.05), and it was significantly lower in the KN93 group than that in the PSD group (P<0.05). The expression of Cx43 mRNA in the PSD and KN93 groups was significantly lower than that in the control group (both P<0.05), and it was significantly higher in the KN93 group than that in the PSD group (P<0.05). *P<0.05, compared with the respective control group; # P<0.05, compared with the respective PSD group. CaMKII, Ca 2+ /CaM-dependent protein kinase II; Cx43, connexin 43; PSD, post-stroke depression.
Article Snippet: TransScript II All-in-One First-Strand cDNA Synthesis SuperMix for PCR and TransScript II SYBR-Green
Techniques: Quantitative RT-PCR, Expressing